Above the wrought-iron screen of this chapel there is a stained-glass window by Enrique Alemán from around 1478. It shows the images of Santa Agueda, Santa Lucia, Santa Cecilia and Santa Ines. Inside, it is illuminated by another stained-glass windows from 1797 representing The Holy Family.

The altar of this chapel stands on a tribune closed off by an iron balustrade. On it there is an important pictorial reredos which was endowed by the canon of the Cathedral, Diego hernandez de Marmolejo, in 1489. The paintings were completed in 1504 and their analysis shows that they are by two artists who have not yet been identified.

As the rest do, the painted panels of the predella depict scenes of The Flagellation, On the way to Calvary, The Crucifixion, The Descent from the Cross, The Pietà. In the first section there are St James, San Blas, San Bartolome, San nicolas and San Sebastian. In the second section finally we find St John the Baptist, Santa Ana, The Virgin with Child, Santa Maria and St Michael.

This reredos has recently been returned to its original state. For this reason the 17C painting of Santa Ana and The Virgin with Child ( a copy the original by Gian Battista Caracciolo in the Museum of Vienna) was removed from the base and taken to the Sacristy of the Chalices.

On the wall facing the screen there is a reredos built in 1914 by joaquin Bilbao with sculptures of The Virgin and St John on the sides. In the centre there is a magnificent painted panel from around 1560 representing a Crucified Christ, which is venerated as the Holy Christ of Maracaibo; its style is close to that of Pedro Villegas Marmolejo.

The wall on the right has the sepulchre of Cardinal Don Luis de la Lastra y Cuesta by the sculptor Ricardo Beller who made it in 1880. The paintings adorning this chapel are an Inmaculada by an anonymous artist imitating Murillo, Abraham and the three Angels ascribed to the 17C Flemish painter Abraham van Diepenbeek, and a figure of Christ served by the Angels, a Seville painting related to the style of Juan de Uceda. Two Flemish copper plates by an anonymous artist, representing The Wedding at Canaab and The Miracle of the loaves and the Fishes round off the decoration of this chapel.